Alpha Fetoprotein |
Leukoagglutinin TestThis test detects leukoagglutinins antibodies that react with white blood cells (WBCs) and may cause a transfusion reaction. These antibodies usually develop after exposure to foreign WBCs through transfusions, pregnancies, and allografts. If a blood recipient has these antibodies, a febrile nonhemolytic reaction may occur 1 to 4 hours after the start of whole blood, red blood cell, platelet, or granulocyte transfusion. This nonhemolytic reaction (marked by fever and severe chills, sometimes with nausea, headache, and transient hypertension) must be distinguished from a true hemolytic reaction before further transfusion can proceed. Clinical Alert: The presence of these antibodies in a recipient can cause immune-mediated platelet refractoriness, a condition characterized by failure of the platelet count to increase after platelet transfusion. The technique used to detect leukoagglutinins is the microlymphocytotoxicity test. In this test, the recipient serum is tested against donor lymphocytes or against a panel of lymphocytes of known HLA phenotype. The antibodies in the recipient serum bind to the corresponding antigen present in the lymphocytes and cause cell membrane injury when the complement is added to the test system. Cell injury is detected by examining the lymphocytes under a microscope. If the lymphocytes don't absorb the added dye, the test is negative. If the lymphocytes show dye uptake, the test is positive. Purpose
Patient preparation
Procedure and posttest care
Precautions
Normal findingsNormally, test results are negative: Agglutination doesn't occur because the serum contains no antibodies. Abnormal findingsA positive result in a transfusion recipient indicates the presence of leukoagglutinins in his blood, identifying his transfusion reaction as a febrile nonhemolytic reaction to these antibodies. A positive result in a donor indicates the presence of leukoagglutinins in his blood, identifying the cause of a recipient's reaction as an acute, noncardiogenic pulmonary edema. If a patient is determined to have antibodies or develops the immune-mediated destruction of transfused platelets, he may require special matched blood products for subsequent transfusions. Interfering factors
|
|
Copyright Diseasesatoz.org All rights reserved.
Bookmark This Page:
Disclaimer: The information contained in this www.diseasesatoz.org web site is not intended as a substitute for medical advice. Everyone's specific situation is different from everyone else's and a health care professional should be consulted about any decision regarding your medical care. We will not be liable for any complications, or other medical accidents arising from the use of any information in this site.